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Acid-Fast Staining Technique
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Acid-Fast Staining "Here’s how to do acid-fast staining. [pause] Start by preparing a thin bacterial smear on a slide, [slight emphasis] air dry it, [pause] and heat-fix gently. [breath] Flood the smear with carbol fuchsin and let it sit for five minutes. [pause] Then, rinse with water and decolorize using acid-alcohol for about 30 seconds, [slight emphasis] rinsing immediately after. [pause] Finally, counterstain with methylene blue for one minute, [pause] rinse, and blot dry. [breath] Acid-fast bacteria will appear bright red, [slight emphasis] and non-acid-fast bacteria will appear blue. [pause] And that’s acid-fast staining in under 50 seconds. [slight emphasis]
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